Plasmid

Part:BBa_J435115:Design

Designed by: Drew Endy, Keith Shearwin   Group: FreeGenes   (2023-05-23)


pOSIP-CH (CamR, HK022)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1125
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3758
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 4802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3758
    Illegal NheI site found at 5093
    Illegal SpeI site found at 1126
    Illegal PstI site found at 1140
    Illegal NotI site found at 1133
    Illegal NotI site found at 4337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3758
    Illegal BglII site found at 469
    Illegal BamHI site found at 22
    Illegal XhoI site found at 1152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1126
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3758
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 4802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 3758
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 4802
    Illegal SpeI site found at 1126
    Illegal PstI site found at 1140
    Illegal AgeI site found at 1343
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 357


Design Notes

Unknown


Source

https://freegenes.github.io/genes/BBF10K_000031.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

References